In biochemical and molecular biology experiments, buffers and surfactants are two important reagents that play a crucial role in maintaining the pH value of biological samples, protecting samples from environmental influences, and promoting interactions between biomolecules. Among them, Tris buffer (trimethylaminomethane buffer) and SDS (sodium dodecyl sulfate) are two commonly used reagents in biochemical research.
tris base powder
Tris base buffer solution
The molecular formula of Tris buffer is C4H11NO3, which is a weakly alkaline buffer. Its molecular structure contains three hydroxide groups, thus exhibiting good buffering performance in the neutral to alkaline range. It is commonly used in biological experiments, such as nucleic acid electrophoresis and protein electrophoresis.
Basic properties of SDS
The molecular formula of SDS is C12H25NaO4S, which is a surfactant and belongs to the category of anionic surfactants. Its long carbon chain allows it to form a micelle structure in water. SDS dissociates into Na+and C12H25OS - in water, and its negative ion can bind to positively charged amino acid residues in proteins, causing them to gain negative charge, undergo denaturation, and exhibit linear conformation.
Preparation and use of Tris buffer solution
Tris buffer is a commonly used running buffer, often prepared together with glycine and SDS to prepare running buffer and loading buffer. The preparation of Tris buffer requires the use of Tris base and hydrochloric acid HCl. According to the required pH value, select the appropriate concentration of Tris base and hydrochloric acid HCl, mix and stir evenly.
Tris buffer is mainly used to maintain pH stability in biochemical experiments. In the processing, separation, and analysis of biological samples, pH is an important environmental factor that affects the structure and activity of biomolecules. Therefore, Tris buffer helps to provide a stable pH environment and protect biomolecules from changes in acidity and alkalinity. In addition, Tris buffer can also serve as a part of the reactants and participate in biochemical reaction processes.
Application of Tris buffer and SDS
1. Nucleic acid electrophoresis: Tris buffer is commonly used as an electrophoresis buffer in nucleic acid electrophoresis, ensuring that an appropriate pH value is maintained during the electrophoresis process, which is conducive to the migration of DNA or RNA. SDS is also used in nucleic acid electrophoresis, but it is usually used as a denaturant for deoxyribonucleic acid (DNA) samples, causing DNA to migrate in a linear state.
2. Protein electrophoresis: Tris buffer is widely used in protein electrophoresis. Its buffering performance enables it to maintain a suitable pH, helping to maintain the natural charge state of proteins. SDS-PAGE (polyacrylamide gel electrophoresis) is often used to separate proteins. SDS can denature proteins and endow them with negative charges, so that their migration speed is linear with molecular weight.
3. Protein dissolution and denaturation: Tris buffer is commonly used for protein dissolution, providing a suitable pH environment. In the process of protein denaturation, the application of SDS can make the protein in a linear state, which is conducive to its migration in gel electrophoresis.
The combined application of Tris buffer and SDS
1. SDS-PAGE: In SDS-PAGE experiments, an electrophoresis buffer containing Tris buffer is usually used to maintain an appropriate pH value. This combination can simultaneously utilize the buffering performance of Tris and the protein denaturation effect of SDS, enabling effective separation of proteins during electrophoresis.
2. Protein precipitation: Tris buffer is also commonly used in combination with SDS for protein precipitation. In some protein extraction experiments, Tris buffer can maintain protein dissolution in cells or tissues, while SDS helps with protein denaturation and dissolution.
Tris buffer and SDS, as commonly used reagents in biochemical experiments, have a wide range of applications in nucleic acid electrophoresis, protein electrophoresis, and protein denaturation and dissolution. Their combined use often better meets the requirements of experiments and improves operational efficiency. Of course, in practical operations, researchers also need to choose appropriate proportions and conditions based on specific experimental requirements, while using high-quality Tris raw materials.
As a biological buffer manufacturer, Desheng has a pure and delicate appearance powder, uniform particles, and good water solubility. Researchers only need to dissolve it to use it without complicated operations. At the same time, the company provides one-on-one service for customers, and supports answering any technical questions at any time. If you are interested in purchasing, please feel free to contact us at any time!