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In the field of chemistry, Tris base is a commonly used laboratory reagent with various applications, including use as a buffer, biochemical experiments, molecular experiments, and so on. Most of the trimethylaminomethane raw materials seen in the market are in powder form, and they need to be prepared into a certain concentration of solution for use in experiments. So how is the operation? What are the specific application areas? Let's take a look below.
Summary of Trihydroxymethyl Aminomethane
Trihydroxymethylaminomethane is an inorganic compound with the molecular formula C4H11NO3 and a molecular weight of 121.135 grams per mole. This is a weakly alkaline molecule, supplied in the form of a solid powder and highly water-soluble. Trihydroxymethylaminomethane is a polyamino alcohol formed by the oxidation of three aminomethane groups with three hydroxyl groups, as shown below:
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Due to its abundant hydroxyl functional groups, trimethylaminomethane has weak alkalinity and buffering properties, making it very useful in biochemical and molecular biology experiments.
Tris base powder
Properties of Trihydroxymethyl Aminomethane Powder
The appearance of trimethylaminomethane powder is a white crystalline powder with the following properties:
(1) High solubility: Trihydroxymethylaminomethane is easily soluble in water, making it an ideal choice for preparing buffer solutions due to its simple preparation and improved efficiency in various experiments.
(2) Weak alkalinity: Trihydroxymethylaminomethane exhibits alkalinity in water, with a pKa value of approximately 6-8, making it suitable for many biochemical and molecular biology applications.
(3) Highly stable: Trihydroxymethylaminomethane powder is relatively stable when placed at room temperature and is not easily decomposed. After being prepared into a solution, the buffering performance is well exerted and is not easily disturbed.
Main uses of trimethylaminomethane powder
1. Preparation of buffer: One of the more common uses of trimethylaminomethane is as a buffer
The composition of the flushing solution. Due to its high solubility and stability, trihydroxymethylaminomethane buffer solution is widely used to regulate the pH value of experimental solutions.
2. Electrophoresis experiment: Tris Borate and Tris Acetate electrophoresis are commonly used buffer systems for DNA and RNA electrophoresis. Trihydroxymethylaminomethane, as a part of the buffer, helps to maintain a constant pH condition and ensure effective separation.
3. Protein electrophoresis: Trihydroxymethyl aminomethane buffer is also used in polyacrylamide gel electrophoresis (PAGE) to separate and analyze proteins.
How to use trimethylaminomethane powder correctly?
1. Accurate weighing: When using trimethylaminomethane powder in the preparation of buffer solutions or other experiments, it is necessary to ensure accurate weighing of the required amount. Generally, a balance is used to accurately weigh to ensure the concentration of the subsequent prepared solution.
2. PH regulation: Trihydroxymethylaminomethane can be used to regulate the pH value of the solution. To accurately adjust the pH value according to experimental needs, hydrochloric acid (HCl) or sodium hydroxide (NaOH) is generally used for pH adjustment.
3. Avoid moisture: Trihydroxymethylaminomethane powder has strong moisture absorption and is very easy to absorb moisture from the air. Therefore, it needs to be stored in a sealed container to avoid moisture.
4. Safe use: When using trimethylaminomethane powder, appropriate safety measures should be taken, including wearing laboratory gloves and goggles to avoid dust inhalation or skin contact.
Trihydroxymethylaminomethane powder is a widely used biological buffer in biochemistry and molecular biology experiments. It has advantages such as high solubility, weak alkalinity, and stability, and is suitable for preparing buffer solutions, electrophoresis experiments, protein analysis, and ion induction experiments. Correct use and handling of it are of great significance to ensure experimental results.
Of course, selecting high-quality raw materials in laboratory work is also a prerequisite. As a manufacturer of trimethylaminomethane, Desheng can supply raw material powders with a purity of up to 99%, and provides one-on-one guidance throughout the entire process. It helps to solve any problems and ensures that customers have no worries about experiments. If you have any interest, please feel free to contact us for purchase!
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