In biomedical research, the collection, transportation, and storage of blood specimens are crucial steps in conducting various biological analyses. To ensure the stability and accuracy of blood samples, the use of anticoagulants and separation gels has become an essential step. Among them, the separated gel tube containing EDTA anticoagulant has become a common choice for venous blood collection, especially in the field of nucleic acid detection. Let's take a look together.
1、 EDTA tube and serum separation gel
EDTA tube is a common blood collection container that contains EDTA anticoagulants and serum separation gel. EDTA salts can bind with calcium ions in the blood to form chelates, preventing blood clotting and ensuring the stability of blood samples. In addition, they can also protect the integrity of nucleic acids, which is beneficial for subsequent nucleic acid testing.
Serum separation gel is a unique biomaterial with good stability and separation performance. When serum separation gel comes into contact with blood, it will automatically separate under the action of centrifugal force based on the specific gravity difference, which can effectively separate serum and blood clots, avoiding their interference in nucleic acid testing experiments. Meanwhile, the addition of serum separation gel can also improve the transportation and storage safety of blood samples.
Serum separation gel
2、 Application of EDTA tube containing serum separation gel in nucleic acid detection
With the development of nucleic acid testing technology, especially the widespread application of real-time fluorescence quantitative PCR technology, higher requirements have been put forward for the collection, transportation, and storage of blood samples. In order to meet these requirements, EDTA tubes containing separated gel are widely used to collect, transport and store venous blood samples for nucleic acid detection.
The EDTA tube containing separating gel can effectively prevent blood coagulation and protect the stability of blood samples, and through the separation of serum separating gel, it can quickly separate the serum and cell components in blood samples, avoid the interference of hemoglobin and other cell components in red blood cells to nucleic acid detection experiments, which greatly improves the accuracy and reliability of nucleic acid detection. At the same time, the EDTA tube containing separated gel can also provide good protection for blood samples and ensure their safety during transportation and storage.
3、 Advantages of EDTA tube containing serum separation gel
EDTA tube containing separation gel plays an important role in nucleic acid detection. By combining the advantages of EDTA anticoagulant and serum separation gel, this tube can provide stable and reliable blood samples to ensure the smooth progress of nucleic acid detection experiments. At the same time, its automatic separation function reduces manual operation steps and improves work efficiency. Due to its excellent stability and safety, the EDTA tube containing separated gel is also suitable for other blood samples requiring long-term storage and transportation.
EDTA potassium salt
4、 Precautions for use of EDTA tube containing serum separation gel
Although the EDTA tube containing separated gel has many advantages, some details should be paid attention to during use, such as following the correct operating steps to avoid violent shaking or tilting the tube, so as to ensure the correct separation of serum and cells. For different types of nucleic acid testing experiments, further optimization of sample processing and preservation methods may be necessary to obtain the best results.
The EDTA tube containing separated gel is an efficient and safe tool for blood sample collection, transportation and storage, which shows unique advantages in the field of nucleic acid detection. Therefore, separated gel and EDTA are also called "ideal partners", which can minimize the interference of hemoglobin and other components on nucleic acid detection.
As a supplier of blood collection tube additives, Desheng can provide high-quality raw materials of EDTA potassium salt and serum separation gel, and with its professional production process and strict quality control system, the supplied blood collection tube additives such as EDTA potassium salt and serum separation gel meet the relevant standards and customer requirements. At the same time, as it is sold by the manufacturer, it not only provides comprehensive after-sales service, but also provides one-on-one assistance to help customers improve experimental efficiency and reduce costs. At present, we have established cooperative relationships with more than 100 blood collection enterprises. If you also have a demand for blood collection additive raw materials, please feel free to click on the website to contact us for purchase!